Identification of a measles virus isolate from a recent outbreak in northern Taiwan

Specimens were collected during a recent outbreak. Those specimens displaying both CPE positive in B95-8 lymphocyte cell culture and positive by IFA were checked by a RT-PCR with a specific set of measles virus primers derived from the C-terminal of the nucleoprotein. Such RT-PCR method was found ideal for routine diagnostic purposes. Product from this RT-PCR was treated for plasmid construction before transformed into E. coli. One of those transformed clones, i. e. T94, was further studied for its DNA sequence. Since T94 is found to bear several evident different characteristics from those ever published, we conclude that this isolate is neither a vaccine derived strain nor one of those reported previously with specific amino acid residues, but unique in its own right. This isolate can well be a local lineage of wild measles virus in Taiwan.