Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypoxia-induced dysfunction in developing rat neocortex

H J Luhmann1, T Kral

  • 1Institute of Neurophysiology, University of Düsseldorf, Germany.

Journal of Neurophysiology
|October 6, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probabilistic graphical model identifies clusters of EEG patterns in recordings from neonates.

Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2019
Same author

Co-activation of VEGF and NMDA receptors promotes synaptic targeting of AMPA receptors.

Molecular psychiatry·2016
Same author

A critical role for VEGF and VEGFR2 in NMDA receptor synaptic function and fear-related behavior.

Molecular psychiatry·2016
Same author

GABA transporters control GABAergic neurotransmission in the mouse subplate.

Neuroscience·2015
Same author

Methylxanthine-evoked perturbation of spontaneous and evoked activities in isolated newborn rat hippocampal networks.

Neuroscience·2015
Same author

Cajal-Retzius cells: update on structural and functional properties of these mystic neurons that bridged the 20th century.

Neuroscience·2014
Same journal

Targeting intracranial electrical stimulation to network regions defined within individuals causes network-level effects.

Journal of neurophysiology·2026
Same journal

When "Noise" Isn't Simply Noise: Deterministic Postural Drive During Noisy Galvanic Vestibular Stimulation (nGVS).

Journal of neurophysiology·2026
Same journal

Abrupt Scene Onsets and Gradually Emerging Scene Information Produce Distinct EEG Decoding Dynamics.

Journal of neurophysiology·2026
Same journal

From discovery to translation: charting a course for the <i>Journal of Neurophysiology</i>.

Journal of neurophysiology·2026
Same journal

Neuromodulatory Strategies Overcome Multiple Inevitable Impairments of Cerebral Palsy.

Journal of neurophysiology·2026
Same journal

Acute Fentanyl Toxicity:From Opioid-Induced to Hypoxia-Mediated Pathophysiology.

Journal of neurophysiology·2026
See all related articles

Young rat cortex shows greater resistance to oxygen deprivation, but prolonged hypoxia causes severe issues. This enhanced sensitivity in immature brains is partly due to N-methyl-D-aspartate receptor activation.

Area of Science:

  • Neuroscience
  • Neurophysiology
  • Developmental Neuroscience

Background:

  • Hypoxia, or oxygen deprivation, poses a significant threat to brain function.
  • The developing brain's response to hypoxic-ischemic events is critical for understanding neurological outcomes.
  • Age-dependent differences in neuronal vulnerability to oxygen deprivation are not fully understood.

Purpose of the Study:

  • To investigate age-related differences in the cortical response to prolonged hypoxia in rats.
  • To characterize the electrophysiological changes, including anoxic depolarization (AD) and calcium dynamics, during hypoxia across different developmental stages.
  • To explore the role of N-methyl-D-aspartate (NMDA) receptors in mediating hypoxic responses in immature versus adult cortex.

Main Methods:

Related Experiment Videos

  • Neocortical slices from young (P5-8), juvenile (P14-18), and adult (>P28) rats were subjected to prolonged hypoxia.
  • Simultaneous measurement of field potential (FP) responses, extracellular DC potential, and extracellular Ca2+ concentration ([Ca2+]o) in somatosensory cortex.
  • Assessment of anoxic depolarization (AD) characteristics (onset latency, amplitude, duration) and recovery of synaptic transmission post-hypoxia.
  • Pharmacological manipulation using ketamine to probe the involvement of NMDA receptors.

    • Main Results:

    • Young and juvenile rat cortex exhibited significantly greater resistance to hypoxia-induced synaptic depression compared to adults.
    • Hypoxia induced anoxic depolarization (AD) with longer latency but greater amplitude and duration in young and juvenile cortex versus adults.
    • Prolonged hypoxia in immature cortex led to a larger decrease in extracellular Ca2+ and, in some cases, spreading depression, with impaired synaptic recovery in adults.

    Conclusions:

    • The immature rat cortex demonstrates a higher tolerance to initial oxygen deprivation but is susceptible to severe pathophysiological events with prolonged hypoxia.
    • Enhanced sensitivity of the immature cortex to severe hypoxia is, at least partly, mediated by N-methyl-D-aspartate receptor activation.
    • Age-dependent differences in hypoxic responses highlight distinct vulnerabilities and resilience mechanisms in the developing versus mature brain.