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The regulation of Streptococcus mutans glucan-binding protein A expression
J A Banas1, H C Potvin, R N Singh
1Department of Microbiology, Immunology, and Molecular Genetics, Albany Medical College, NY 12208, USA. Jeff_Banas@CCGATEWAY.AMC.EDU
Abstract:
The S. mutans GBP-A is hypothesized to be constitutively expressed and to contribute to the sucrose-dependent colonization of S. mutans. To investigate GBP-A expression, a reporter gene encoding chloramphenicol acetyltransferase (CAT) was placed downstream of the gbpA promoter and CAT activity was measured under conditions that would be associated with the sucrose-dependent colonization of S. mutans. Expression of GBP-A was optimal under anaerobiosis and neutral pH conditions, and correlated with optimal growth. The addition of sucrose to the growth medium did not elevate the expression of GBP-A.