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[Serum apolipoprotein E-rich HDL-C levels in the subjects with hyperalphalipoproteinemia]
1Department of Laboratory Medicine, Niigata University School of Medicine.
To clarify an increasing factor of serum apolipoprotein E-rich HDL-C(apo E-rich HDL-C) level in the patients with hyperalphalipoproteinemia(HALP), we characterized the relationships between the serum apo E-rich HDL-C levels and, apolipoprotein E genotypes and cholesteryl ester transfer protein(CETP) gene mutations using healthy(n = 46) and HALP subjects(HDL-C > or = 100 mg/dl, n = 67). Serum apo E-rich HDL-C level of healthy subjects was significantly different from that of HALP subjects. Frequencies of apo E allele of all subjects studied were consistent with previous other reports. It was, however, demonstrated that apo epsilon 4 allele decreases the apo E-rich HDL-C level in all HALP subjects. On the other hand, the G to A mutation in the intron 14 splice donor site(114A) and Asp442 to Gly mutation in the exon 15(D442G), were analysed by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) method. Then we compared the apo E-rich HDL-C levels between the HALP subjects in the almost general population of apo E 3/3 with and without CETP gene mutation. There was strong tendency that the existence of CETP mutations increased the serum apo E-rich HDL-C level, comparing with the HALP subjects without these mutations. Therefore, we suggested that the increasing of serum apo E-rich HDL-C level was caused by the CETP deficiency and its level was affected by apo E genotypes.
To clarify an increasing factor of serum apolipoprotein E-rich HDL-C(apo E-rich HDL-C) level in the patients with hyperalphalipoproteinemia(HALP), we characterized the relationships between the serum apo E-rich HDL-C levels and, apolipoprotein E genotypes and cholesteryl ester transfer protein(CETP) gene mutations using healthy(n = 46) and HALP subjects(HDL-C > or = 100 mg/dl, n = 67). Serum apo E-rich HDL-C level of healthy subjects was significantly different from that of HALP subjects. Frequencies of apo E allele of all subjects studied were consistent with previous other reports. It was, however, demonstrated that apo epsilon 4 allele decreases the apo E-rich HDL-C level in all HALP subjects. On the other hand, the G to A mutation in the intron 14 splice donor site(114A) and Asp442 to Gly mutation in the exon 15(D442G), were analysed by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) method. Then we compared the apo E-rich HDL-C levels between the HALP subjects in the almost general population of apo E 3/3 with and without CETP gene mutation. There was strong tendency that the existence of CETP mutations increased the serum apo E-rich HDL-C level, comparing with the HALP subjects without these mutations. Therefore, we suggested that the increasing of serum apo E-rich HDL-C level was caused by the CETP deficiency and its level was affected by apo E genotypes.