Experimental induction of bacillary hemoglobinuria in cattle

An open-surgery technique for intrahepatic inoculation of Clostridium haemolyticum spores, suspended in calcium chloride as the hepatic debilitant, was used to produce bacillary hemoglobinuria in cattle. All calves (n=3) died of the disease, and the controls (n=2) given calcium chloride without spores survived. Clinical signs and gross pathologic changes produced by this method resembled those described for the disease in its natural form.