Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[New quantitative method for determining p53 protein in bladder carcinomas]

J Moreno Sierra1, M L Maestro de las Casas, M D Ortega Heredia

  • 1I Cátedra de Urología, Hospital Universitario San Carlos., Madrid, España.

Archivos Espanoles De Urologia
|May 1, 1997
PubMed
Summary

Elevated protein p53 levels in bladder tumors indicate more aggressive disease. This new quantitative method helps identify patients with higher-risk bladder cancer for targeted treatment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial comment on the article «Perioperative and periprocedural management of antithrombotic therapy: 2025 consensus document of SEC, SEDAR, SEACV, SECCE, AEC, SECOM CYC, SECPRE, SEPD, SEGG, SEGO, SEHH, SETH, SEMERGEN, SEMFYC, SEMG, SEMICYUC, SEMI, SEMES, SEN, S.E.N., SENEC, SEPAR, SEO, SEORL-CCC, SEPA, SERVEI, SECOT, and AEU».

Actas urologicas espanolas·2026
Same author

Prospective analysis of urinary continence and urethral stricture after Holmium laser enucleation of the prostate (HoLEP): A consecutive 254-case series.

Actas urologicas espanolas·2026
Same author

Prospective analysis of the learning curve in holmium laser enucleation of the prostate (HoLEP): A 125 case series.

Actas urologicas espanolas·2025
Same author

Protocol description and initial experience in kidney graft perfusion using infrared thermography.

World journal of urology·2024
Same author

[Evaluation of testicular growth after varicocele treatment in early childhood and adolescence based on the technique used (Palomo, Ivanissevich and embolization)].

Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica·2017
Same author

Other radiological lesions of the Lower Urinary Tract in patients after isolated pelvic radiotherapy and combined with surgery.

Archivos espanoles de urologia·2016

Area of Science:

  • Oncology
  • Molecular Biology
  • Uropathology

Background:

  • The molecular biology of bladder tumors is complex, with alterations in protein p53 expression potentially playing a significant role.
  • Understanding p53 expression is crucial for determining tumor behavior and patient prognosis.

Purpose of the Study:

  • To analyze protein p53 expression in bladder tumors using a novel quantitative immunoluminescence assay.
  • To investigate the biological implications of altered p53 expression in transitional cell carcinoma of the bladder.

Main Methods:

  • A quantitative immunoluminescence assay (LIA-mat p53 method) was employed to measure protein p53 levels.
  • The study included 60 patients with bladder transitional cell carcinoma and 14 healthy urothelial tissue controls.

Related Experiment Videos

Main Results:

  • Protein p53 oncoprotein levels were significantly higher in bladder carcinoma tissues compared to healthy urothelial tissues.
  • Elevated p53 expression correlated with infiltrating and undifferentiated tumors, as well as with patient mortality.

Conclusions:

  • Quantitative determination of protein p53 expression using the LIA-mat method effectively identifies subgroups of bladder cancer patients with more aggressive tumor behavior.
  • This method aids in predicting prognosis and potentially guiding therapeutic strategies for bladder cancer.