Ethanol effects on postimplantation rat embryos: influence of zinc and methionine

Organic zinc salts and thiols, administered simultaneously, protect mice synergistically against ethanol toxicity. Moreover, chronic ethanol consumption could affect the bioavailability of zinc and amino acids such as methionine. This could result in impaired embryonic growth and development. The influence of zinc and methionine on ethanol-induced embryopathy was investigated by simultaneous administration of ethanol, zinc and methionine to pregnant rats from gestational day 6 through 12. Ethanol was given in the form of a liquid diet; zinc administered i.p., and methionine was given by gavage. The ethanol group received the liquid ethanol diet; the ethanol + zinc and methionine group received the ethanol diet, zinc and methionine; and the pair-fed control group was given an isocaloric control diet. On day 12 of gestation, embryos of ethanol alone treated rats revealed a significantly reduced embryonic protein content, number of somites, crown-rump length, and lower morphological score (embryological differentiation) compared to the pair-fed control embryos. However, embryonic growth and developmental parameters in the ethanol, zinc and methionine treated group were not significantly different from those exposed to ethanol alone.