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Related Experiment Videos

Initiation codon scanthrough versus termination codon readthrough demonstrates strong potential for major

T N Bullock1, A E Patterson, L L Franlin

  • 1Department of Microbiology and Immunology, Kimmel Cancer Center, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USA.

The Journal of Experimental Medicine
|October 7, 1997
PubMed
Summary

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This summary is machine-generated.

Scanthrough translation allows cryptic epitopes from alternative reading frames to be expressed. This mechanism significantly impacts CD8(+) T cell responses and tolerance induction.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Major histocompatibility complex class I-restricted epitopes can originate from non-conventional translation.
  • Scanthrough translation, bypassing the primary start codon, has been implicated in cryptic epitope presentation from alternative reading frames.

Purpose of the Study:

  • To confirm and extend findings on scanthrough translation's role in cryptic epitope expression.
  • To investigate the efficiency of eukaryotic translation termination codons in preventing epitope expression from 3' untranslated regions.

Main Methods:

  • Utilized an epitope cassette construct with two CD8(+) T cell epitopes in alternative reading frames.
  • Assessed the impact of initiation codon context, start codon number, and epitope generation efficiency on scanthrough translation.

Related Experiment Videos

  • Investigated eukaryotic stop codon efficiency for epitope expression from 3' untranslated regions.

    • Main Results:

    • Scanthrough translation demonstrated high potency in the experimental system.
    • Epitope expression via scanthrough was modulated by initiation codon context and preceding start codons.
    • Eukaryotic stop codons were highly efficient, preventing epitope expression from 3' untranslated regions.

    Conclusions:

    • Scanthrough translation is a potent mechanism for expressing epitopes from upstream alternative open reading frames.
    • This mechanism likely contributes significantly to CD8(+) T cell responses and immune tolerance induction.
    • 3' untranslated regions are unlikely to be a common source of cryptic epitope substrates.