Regulation of insulin-like growth factor-binding protein-1 and progesterone secretion from human granulosa-luteal cells: effects of octreotide and insulin
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N Holst1, K H Kierulf, M Seppälä
1Department of Obstetrics and Gynecology, Ulleval Hospitals, University of Oslo, Norway.
Objective:
To examine the effects of the synthetic somatostatin-analogue octreotide and human recombinant insulin on the release of insulin-like growth factor binding protein-1 (IGFBP-1) and P from human granulosa-luteal cells.
Design:
Primary culture of human granulosa-luteal cells.
Setting:
Academic research laboratory.
Patient(S):
Women undergoing oocyte retrieval for IVF-ET because of tubal infertility.
Intervention(S):
Octreotide or insulin were added to the cultures; sampling of culture medium was performed after 48 hours.
Main Outcome Measure(S):
Insulin-like growth factor binding protein-1 and P.
Result(S):
Octreotide significantly inhibited IGFBP-1 (58.8% compared with controls) and P release (66.1% compared with controls). Insulin abolished IGFBP-1 release while stimulating P release (200.7% compared with controls). There was a significant and positive correlation between IGFBP-1 and P levels.
Conclusion(S):
Octreotide and insulin have a significant effect on human granulosa-luteal cell function in terms of IGFBP-1 and P release. Our results suggest a local ovarian mechanism for the recently observed effects of octreotide in the treatment of women with polycystic ovary syndrome.