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Detrimental effect of hydrosalpinx fluid on the development and blastulation of mouse embryos in vitro
R Sachdev1, E Kemmann, M K Bohrer
1Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey (UMDNJ)-Robert Wood Johnson Medical School, New Brunswick 08901, USA.
Objective:
To study the effects of human hydrosalpinx fluid on mouse embryo blastulation rate.
Design:
Comparison of mouse embryo blastulation rate in media containing increasing concentrations of hydrosalpinx fluid.
Setting:
Tertiary care center.
Patient(S):
Women undergoing laparoscopic evaluation or treatment for infertility noted to have hydrosalpinx or paratubal cyst.
Intervention(S):
Exposure of mouse embryos to hydrosalpinx or paratubal cyst fluid collected during laparoscopy.
Main Outcome Measure(S):
Blastulation rate of mouse embryos.
Result(S):
Culture of mouse embryos at 0% (controls), 0.3%, 0.6%, and 0.9% hydrosalpinx fluid concentrations demonstrated significantly lower blastulation rate at each level compared with the controls.
Conclusion(S):
Hydrosalpinx fluid is highly embryotoxic.