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Resazurin reduction test to evaluate semen quality

K V Reddy1, P K Meherji, J S Gokral

  • 1Institute for Research in Reproduction (ICMR), Mumbai, India.

Indian Journal of Experimental Biology
|April 1, 1997
PubMed
Summary
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The resazurin test (REZ TEST) offers a simple, equipment-free method to assess semen quality. It strongly correlates with sperm motility, viability, and concentration, aiding in the evaluation of male fertility.

Area of Science:

  • Reproductive Biology
  • Andrology
  • Clinical Chemistry

Background:

  • Male infertility affects a significant portion of couples seeking reproductive assistance.
  • Accurate and accessible semen analysis is crucial for diagnosing male factor infertility.
  • Current semen analysis methods can be labor-intensive and require specialized equipment.

Purpose of the Study:

  • To evaluate the efficacy of the resazurin test (REZ TEST) as a simple diagnostic tool for assessing semen quality.
  • To compare the results of the REZ TEST with established semen parameters such as sperm motility, viability, and concentration.
  • To determine the diagnostic accuracy and predictive values of the REZ TEST in distinguishing between fertile and subfertile males.

Main Methods:

  • The resazurin test (REZ TEST) was conducted on semen samples from 75 untreated subfertile males and 10 fertile males.

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  • REZ TEST results were correlated with standard semen parameters including sperm motility, viability, and concentration.
  • Statistical analysis was performed to assess the correlation coefficients and predictive values of the REZ TEST.

    • Main Results:

    • The REZ TEST demonstrated a high correlation with motile sperm (r = 0.90), sperm viability (r = 0.80), and sperm concentration (r = 0.71).
    • Significant differences in positive test rates were observed between fertile males and patients with abnormal semen parameters.
    • The REZ TEST showed a positive predictive value of 93.75% for progressive motile sperm concentration > 20 x 10^6/ml and a negative predictive power of 88.7% for concentration > 10 x 10^6/ml.

    Conclusions:

    • The resazurin test (REZ TEST) is a reliable and accurate method for assessing semen quality.
    • The REZ TEST is a valuable tool for evaluating male fertility due to its strong correlation with key sperm parameters.
    • Its ease of performance, minimal sample volume requirement, and lack of need for specialized equipment make the REZ TEST a practical option for clinical use.