Transient alteration of the reproductive function in nephrotic rats

The reproductive function of male and female rats with induced nephrotic syndrome was examined by assessing hormonal levels, the estrous cycle pattern and fertility. Measurements were carried out on day 10 (nephrotic stage) and on day 30 (remission stage) after treatment (a single s.c. dose of puromycin aminonucleoside 15 mg/100 g body wt.). Serum concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and progesterone (P4) from both sexes as well as 17beta-estradiol (E2) in females and total testosterone (tT) in males were assayed at both phases of the illness by specific radioimmunoassays; free and urine testosterone (fT, uT) was also determined in male rats on day 10. The estrous cycle was followed alongside the study through vaginal smears, while fertility was estimated by the rate of pregnancies (females) or pregnancy induction (males), and by the litter size. The results showed that most serum hormone levels, except FSH in females, were significantly reduced at the nephrotic stage of the illness, returning to their normal values after 30 days. Besides, on day 10, fT was found reduced in nephrotic males, while uT concentrations remained unmodified. The ovulatory cycle of nephrotic rats was disrupted on day 3 and not restored until day 23; nevertheless, the reproductive function, measured as a fertility index, was fully reinstalled at the remission stage. Interestingly, the capacity of fertilization of the nephrotic males was not entirely abolished during the acute phase, since 37% of the animals were fertile. Moreover, on day 30 the reproductive function in nephrotic males was totally recovered. The data from this study show the existence of reversible endocrine disorders in rats bearing nephrotic syndrome; such changes are manifested during the acute phase of the illness as a temporary cessation of the reproductive processes.