Related Experiment Video
Updated: Aug 14, 2026

RhoC GTPase Activation Assay
Published on: August 22, 2010
Transcriptional regulation of the Ras-related protein TC21/R-Ras2 in endothelial cells
1Department of Gynecology and Obstetrics, University of Göttingen Medical School, Germany.
Abstract:
Applying an RNA display strategy to identify genes of autocrine activated endothelial cells, we identified among others the Ras-related protein TC21/R-Ras2 as a differentially expressed gene of bovine aortic endothelial cells (BAEC). Migrating BAEC express prominently upregulated steady state levels of TC21/R-Ras2 mRNA (Northern blot, in situ hybridization) and protein (Western blot). Growth factor stimulation identified TC21/R-Ras2 as aFGF, bFGF, and EGF inducible molecule of BAEC. Exposure to actinomycin D revealed a half life time of TC21/R-Ras2 mRNA of > 2 h. These results strongly suggest that transcriptional regulation of Ras molecules contributes to their signal transduction capacity and a possible role of TC21/R-Ras2 in the signal transduction of autocrine activated endothelial cells.
More Related Videos
Related Concept Videos
Master Transcription Regulators
The Ras Gene
Ras is a superfamily...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Regulation of Angiogenesis and Blood Supply
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
MAPK Signaling Cascades

