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Related Experiment Videos

Calbindin-D28k in nerve cell nuclei

D C German1, M C Ng, C L Liang

  • 1Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas 75235-9070, USA.

Neuroscience
|October 8, 1997
PubMed
Summary
This summary is machine-generated.

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Calbindin-D28k, a calcium-binding protein, is found in the nucleus of specific neurons. This nuclear presence has significant implications for how cells regulate gene expression in response to calcium signals.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Calbindin-D28k is an EF-hand calcium-binding protein.
  • It is known to buffer cytosolic calcium and protect cells from calcium-induced degeneration.
  • Its presence in the neuronal nucleus has been uncertain.

Purpose of the Study:

  • To investigate the subcellular localization of Calbindin-D28k in the nervous system.
  • To determine if Calbindin-D28k is present in the nucleus of specific neuronal populations.

Main Methods:

  • Light and electron microscopic immunohistochemistry.
  • Nuclear fractionation techniques.

Main Results:

  • Calbindin-D28k was localized to both the cytoplasm and nucleus of rodent midbrain dopaminergic neurons and cerebellar Purkinje cells.

Related Experiment Videos

  • Nuclear staining intensity for Calbindin-D28k was equal to or greater than cytoplasmic staining.

    • Conclusions:

    • Calbindin-D28k is present in the nucleus of key neuronal types.
    • Nuclear Calbindin-D28k has potential roles in regulating gene expression in response to nuclear calcium signaling.