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Related Experiment Videos

Citrate metabolism by human platelets

S Cartledge1, D J Candy, R J Hawker

  • 1School of Biochemistry, University of Birmingham, Edgbaston, UK.

Transfusion Medicine (Oxford, England)
|October 8, 1997
PubMed
Summary
This summary is machine-generated.

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Platelets metabolize citrate, an anticoagulant, at low rates in artificial media. Citrate levels during storage do not activate platelets, ensuring transfusion safety.

Area of Science:

  • Biochemistry
  • Hematology
  • Cellular Metabolism

Background:

  • Citrate is a common anticoagulant in platelet storage media.
  • Citrate is also an intermediate in cellular oxidative metabolism.
  • Understanding citrate metabolism in platelets is crucial for assessing storage conditions.

Purpose of the Study:

  • To quantify the metabolic utilization of citrate by human platelets in artificial media.
  • To determine if citrate metabolism can lead to platelet activation under storage conditions.

Main Methods:

  • Human platelets were isolated and incubated with [14C]citrate in a defined medium at 22°C.
  • Production of labeled carbon dioxide was measured over 2-hour incubations.
  • Citrate oxidation rates were assessed with and without an alternative substrate (acetate).

Related Experiment Videos

  • Conversion of citrate to glycogen was also evaluated.

    • Main Results:

    • Citrate was oxidized to carbon dioxide by platelets at a significant, albeit low, rate (0.3 nmol/10(9) platelets/h).
    • Citrate oxidation was reduced in the presence of acetate, indicating substrate competition.
    • No significant conversion of citrate to glycogen was observed.
    • Calculated citrate consumption under normal storage conditions is insufficient to cause platelet activation.

    Conclusions:

    • Platelet metabolism of citrate is minimal and does not lead to significant citrate depletion.
    • Current storage conditions and citrate concentrations are unlikely to induce platelet activation via citrate metabolism.
    • These findings support the safety of using citrate as an anticoagulant in platelet concentrates for transfusion.