Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Process control of filtered red blood cells: which counting method?

R Conte1, A Bontadini, D Cirillo

  • 1Servizio di Immunoematologia e Trasfusionale, Policlinico S. Orsola-Malpighi, Bologna, Italy.

Transfusion Medicine (Oxford, England)
|October 8, 1997
PubMed
Summary

Accurate white blood cell (WBC) counting is crucial for filtered blood components. This study compared four methods, finding significant differences in residual WBC counts, impacting process control decisions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experiences from 4 Years of Organization of an External Quality Assessment for Mycobacterium tuberculosis Whole-Genome Sequencing in the European Union/European Economic Area.

Microbiology spectrum·2022
Same author

Epidemiology and treatment outcome of MDR and pre-XDR TB in international migrants at two reference centers in the North of Italy: a cross-sectional study coordinated by Stop TB Italia Onlus.

Public health·2019
Same author

Satisfaction grade assessment of patients treated with zygomatic implants with self-tapping apex and machined body.

Journal of biological regulators and homeostatic agents·2019
Same author

Tuberculosis screening in outpatient healthcare workers: lessons from a high-income, low TB burden country.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2019
Same author

Comparison of the DNA Content, Bromodeoxyuridine Incorporation and Ki-67 Antigen Expression in Human Acute Myeloid Leukemia.

Leukemia & lymphoma·2016
Same author

Clinical implications of molecular drug resistance testing for Mycobacterium tuberculosis: a TBNET/RESIST-TB consensus statement.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2015

Area of Science:

  • Hematology
  • Biotechnology
  • Medical Laboratory Science

Background:

  • High-efficiency filters reduce white blood cells (WBCs) in blood components.
  • Accurate enumeration of residual WBCs is essential for process control.
  • Existing counting methods vary in sensitivity and feasibility.

Purpose of the Study:

  • To compare the sensitivity and feasibility of four different methods for counting residual white blood cells (WBCs) in leuco-depleted blood components.
  • To determine the most efficient laboratory method for process control of WBC-reduced packed red blood cells.
  • To address the need for sensitive methods to quantify low concentrations of WBCs post-filtration.

Main Methods:

  • Comparison of four WBC counting methods: Nageotte chamber analysis, flow cytometry, the fluorochrome method (Borzini), and modified Nageotte chamber analysis (Prati).

Related Experiment Videos

  • Assessment of sensitivity and laboratory feasibility for each method.
  • Evaluation of residual WBC content in filtered blood units using the different counting techniques.

    • Main Results:

    • Observed significant differences in post-filtration WBC content based on the counting method employed.
    • Demonstrated variability in the sensitivity of the evaluated methods for low WBC concentrations.
    • Highlighted the impact of counting method choice on the assessment of WBC reduction efficacy.

    Conclusions:

    • The choice of WBC counting method significantly influences the determination of residual white blood cell levels in filtered blood components.
    • A method's sensitivity must be appropriate for the desired goal, especially with high-efficiency filtration.
    • Standardization of WBC counting methods is necessary for reliable process control across multiple laboratories.