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Immunomagnetic separation and PCR for detection of Mycobacterium ulcerans
1Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville, Australia. Brenda.Roberts@jcu.edu.au
Journal of Clinical Microbiology
|October 8, 1997
Abstract:
We have developed a technique based on the use of monodisperse magnetic beads to isolate Mycobacterium ulcerans from heterogenous mixtures, prior to PCR amplification. Using this method, we were able to detect M. ulcerans in water samples taken from Phillip Island, Australia, the site of several outbreaks of M. ulcerans disease in recent times.