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Enterococcus faecalis antigens in human infections

Y Xu1, L Jiang, B E Murray

  • 1Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77030, USA.

Infection and Immunity
|October 8, 1997
PubMed
Summary
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Methods in molecular medicine·2011

Researchers screened genomic libraries of Enterococcus faecalis strains for proteins recognized by patient sera. This study identified potential virulence factors and novel genes, advancing our understanding of enterococcal infections.

Area of Science:

  • Microbiology
  • Genomics
  • Immunology

Background:

  • Enterococcus faecalis is a significant cause of hospital-acquired infections, particularly endocarditis.
  • Identifying E. faecalis virulence factors is crucial for developing targeted therapies and diagnostics.

Purpose of the Study:

  • To construct and screen genomic libraries of E. faecalis strains for immunogenic proteins.
  • To identify novel genes and potential virulence factors associated with E. faecalis endocarditis.

Main Methods:

  • Genomic libraries of E. faecalis strains OG1RF and TX52 were created using cosmid vectors.
  • Libraries were screened using sera from patients with enterococcal endocarditis and a rabbit immunized with E. faecalis surface proteins.
  • Immunopositive clones were subcloned, sequenced, and analyzed for similarities to known proteins.

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Main Results:

  • Seventy-five cosmid clones showed reactivity with patient sera.
  • Thirty-eight distinct immunopositive clones were further analyzed, with their sequences revealing similarities to known virulence factors, transporters, and regulatory proteins.
  • Fourteen subclones exhibited no significant database similarity, suggesting the presence of novel genes. One clone produced a nonprotein antigen.

Conclusions:

  • This study successfully identified immunogenic E. faecalis proteins and potential novel genes using genomic screening.
  • The findings contribute to understanding the molecular basis of E. faecalis pathogenesis and host immune response.
  • Identified antigens may serve as targets for future diagnostic or therapeutic strategies against enterococcal infections.