Solubility of 92 in blood with different hemoglobin concentrations at low temperatures

The Brunsen solubility coefficients for O2 in blood obtained from healthy nonsmoking subjects have been determined at 0, 6, and 12 degrees C for bloods of hemoglobin concentrations 5.6, 10.2, 15.7, and 22.5 g/100 ml. Direct gasometric measurements show that blood has a relative solubility (alphabeta/alphah2o) different for each hemoglobin concentration but constant for any given hemoglobin concentration at the temperatures studied (0.6, and 12 degrees C). For hemoglobin concentrations 5.6, 10.2, 15.7, and 22.5 g/100 ml alphabetao degrees C/alphabeta 12 degrees C rations were 1.36, 1.35, 1.35, and 1.35, respectively. The alpha H20(0 degrees C)/alpha H20(12 degrees C) ratio is 1.34. Consequently, it was possible to construct a table for the Brunsen solubility coefficients for O2 in blood from O to 15 degrees C of different hemoglobulin concentrations. Values from 10 to 15 degrees C published in the Biologal Hanbook, Respiration and Circulation, based on extrapolations from previous direct measurements of temperatures from 18 to 37 degrees C differ only 1% from our present results.