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Related Experiment Videos

Multiple high-affinity binding sites for

Winberg, Nilsson

    The Journal of Experimental Biology
    |January 1, 1996
    PubMed
    Summary
    This summary is machine-generated.

    This study characterizes serotonin (5-HT) binding sites in Arctic charr brains, revealing at least three distinct high-affinity sites. One site closely resembles the mammalian 5-HT1A receptor, offering insights into fish neurochemistry.

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    Area of Science:

    • Neuroscience
    • Pharmacology
    • Ichthyology

    Background:

    • Serotonin (5-HT) is a crucial neurotransmitter involved in various physiological processes.
    • Understanding serotonin receptor subtypes in fish is essential for comparative neurobiology.
    • Arctic charr (Salvelinus alpinus) brain homogenates were used to investigate 5-HT binding characteristics.

    Purpose of the Study:

    • To characterize the binding kinetics and pharmacology of serotonin (5-HT) binding sites in Arctic charr brain.
    • To identify potential serotonin receptor subtypes present in Arctic charr.
    • To investigate the influence of guanosine triphosphate (GTP) on 5-HT binding site characteristics.

    Main Methods:

    • Radioligand binding assays using [3H]serotonin (5-HT) to Arctic charr brain membranes.

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  • Saturation binding experiments to determine dissociation constant (KD) and maximum binding capacity (Bmax).
  • Displacement studies using various 5-HT receptor ligands (8OH-DPAT, buspirone, TFMPP, spiperone, mianserin) and GTP.

    • Main Results:

    • Binding of [3H]5-HT was best described by a one-site model with KD = 5.7±0.3 nmol l-1 and Bmax = 60.7±7.3 fmol mg-1 protein.
    • Ligand displacement studies revealed multiple binding sites: 8OH-DPAT interacted with three sites, while buspirone, TFMPP, spiperone, and mianserin identified two sites.
    • GTP reduced the Bmax of high-affinity sites by 60% for 8OH-DPAT and 50% for [3H]5-HT saturation, suggesting involvement of G-protein-coupled receptors, though 8OH-DPAT still differentiated three sites.

    Conclusions:

    • Arctic charr brain possesses at least three distinct high-affinity [3H]5-HT binding sites.
    • One of these sites exhibits a pharmacological profile highly similar to the mammalian 5-HT1A receptor.
    • The findings provide valuable insights into the neurochemical makeup of fish serotonin systems and their evolutionary conservation.