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Left ventricular volumes and function in the embryonic mouse heart

N Tanaka1, L Mao, F A DeLano

  • 1Department of Medicine, University of California San Diego, La Jolla 92093-0613, USA.

The American Journal of Physiology
|October 10, 1997
PubMed
Summary

Researchers developed miniaturized technology to analyze embryonic mouse heart function in vivo. This technology revealed significant increases in left ventricle (LV) volume and ejection fraction from embryonic day 10.5 to 14.5.

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Area of Science:

  • Developmental Biology
  • Cardiovascular Research
  • Bioengineering

Background:

  • Assessing embryonic cardiac function in vivo is crucial for understanding heart development and disease.
  • Existing methods often lack the resolution or invasiveness required for detailed embryonic cardiac analysis.

Purpose of the Study:

  • To describe novel miniaturized technology for in vivo analysis of embryonic mouse heart volume and function.
  • To apply this technology to study the normal embryonic left ventricle (LV) at two developmental stages.

Main Methods:

  • Microsurgical techniques were used to isolate embryos from embryonic day (ED) 10.5 to 16.
  • Intravital microscopy with transillumination was employed for cardiac visualization, with intact umbilical circulation.
  • Imaging techniques evolved from color visualization at ED10.5-11 to fluorescent imaging at ED13.5-14.5.

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Main Results:

  • Left ventricle (LV) end-diastolic volumes averaged 0.16 µL at ED10.5-11 and 0.57 µL at ED13.5-14.5.
  • A positive linear relationship was observed between LV end-diastolic volume and stroke volume.
  • Average LV ejection fractions were 27.4% at ED10.5-11 and 58.4% at ED13.5-14.5, approaching adult levels by ED13.5-14.5.

Conclusions:

  • The developed miniaturized technology enables effective in vivo assessment of embryonic mouse cardiac function.
  • This technology is valuable for studying cardiac development and identifying abnormalities in genetically modified mouse models.
  • The findings provide normative data for embryonic mouse LV function at key developmental time points.