Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chloroplastic prenylated proteins

I Parmryd1, C A Shipton, E Swiezewska

  • 1Department of Biochemistry, Stockholm University, Sweden. ingela@biokemi.su.se

FEBS Letters
|October 10, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mild experimental exacerbation of asthma induced by individualised low-dose repeated allergen exposure. A double-blind evaluation.

Respiratory medicine·1999
Same author

Fluoride augments the mitogenic and antigenic response of human blood lymphocytes in vitro.

Caries research·1999
Same author

[Health care and patients' need for emergency medicine].

Lakartidningen·1998
Same author

The NIK protein kinase and C17orf1 genes: chromosomal mapping, gene structures and mutational screening in frontotemporal dementia and parkinsonism linked to chromosome 17.

Human genetics·1998
Same author

2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803.

FEBS letters·1998
Same author

Five-year prospective study of prosthodontic and surgical single-tooth implant treatment in general practices and at a specialist clinic.

The International journal of prosthodontics·1998

This study provides evidence for protein prenylation in spinach chloroplasts, identifying prenylated polypeptides and their membrane localization. Farnesyl protein transferase activity was detected in the thylakoid membrane.

Area of Science:

  • Plant molecular biology
  • Chloroplast biochemistry
  • Protein modification

Background:

  • Protein prenylation is a crucial post-translational modification.
  • Its presence and function in plant chloroplasts remain largely unexplored.

Purpose of the Study:

  • To investigate the existence and characteristics of protein prenylation within spinach chloroplasts.
  • To identify prenylated proteins and their subcellular localization.

Main Methods:

  • In vivo [3H]mevalonate labeling of spinach plants.
  • Biochemical analysis including SDS-PAGE and autoradiography.
  • Thermolysin treatment of intact chloroplasts to assess surface localization.

Main Results:

Related Experiment Videos

  • Approximately 20 prenylated polypeptides were identified in chloroplasts.
  • 40% of these were localized to the cytoplasmic surface of the outer envelope membrane.
  • The remaining prenylated polypeptides were found in thylakoids and/or the inner envelope membrane, often within larger protein complexes.
  • Farnesyl protein transferase activity was associated with the thylakoid membrane.

    • Conclusions:

    • Protein prenylation occurs in spinach chloroplasts, involving multiple polypeptides.
    • Prenylated proteins are distributed across different chloroplast compartments, including envelope membranes and thylakoids.
    • The presence of farnesyl protein transferase activity suggests a functional prenylation pathway within the thylakoid membrane.