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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Alpha-tocopherol does not inhibit hypochlorite-induced oxidation of apolipoprotein B-100 of low-density lipoprotein
1The Heart Research Institute, Camperdown, NSW, Australia.
Abstract:
The amount of alpha-tocopherol (alpha-TOH) can dramatically alter the extent of radical-induced oxidation of low density lipoprotein (LDL) lipids, a process generally thought to be important in atherogenesis. However, LDL with atherogenic features can also be formed in vitro by exposure to the strong non-radical oxidant hypochlorite (HOCl), which preferentially oxidises LDL apolipoprotein B-100. Here we show that varying LDL content of alpha-TOH by vitamin supplementation or depletion has no effect on the extent of HOCI-induced oxidation of apolipoprotein B-100 as measured by the loss of lysine and tryptophan residues, and the alteration in relative electrophoretic mobility of the lipoprotein particle.
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