Enantioselective analysis of praziquantel in plasma samples
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Published on: October 11, 2019
V A Jabor1, G M Rocha, P S Bonato
1Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Brazil.
Abstract:
A direct enantioselective high-performance liquid chromatography method is described for the quantitative determination of praziquantel enantiomers in plasma samples. The method involves two-step extraction of plasma with toluene, evaporation of the solvent and chromatography on a Chiralcel OD-H column using hexane-ethanol (85:15, v/v) as the mobile phase and detection at 220 nm. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.
