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Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
06:40

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Published on: July 28, 2023

Helicobacter pylori infection in children in Saudi Arabia

A M Abdullah1, M O Gad el Rab, I al Ayed

  • 1Department of Paediatrics, Faculty of Medicine, King Saud University, Riyadh, Saudi Arabia.

Tropical Gastroenterology : Official Journal of the Digestive Diseases Foundation
|April 1, 1997
PubMed

Insights

Serology is an effective diagnostic tool for identifying Helicobacter pylori (H. pylori) infections in children. This study in Saudi Arabia found antibody tests reliable for diagnosing H. pylori in pediatric patients.

Area of Science:

  • Pediatric Gastroenterology
  • Infectious Diseases
  • Diagnostic Immunology

Background:

  • Helicobacter pylori (H. pylori) infection is a significant cause of pediatric gastrointestinal disease.
  • Accurate and accessible diagnostic methods are crucial for timely H. pylori treatment in children.

Purpose of the Study:

  • To evaluate the diagnostic utility of serological tests for H. pylori infection in a pediatric cohort.
  • To correlate serological findings with microbiological and histopathological results.

Main Methods:

  • A cohort of 20 children with suspected H. pylori infection was studied.
  • H. pylori IgG and IgA antibody levels were measured via serology.
  • Gastric and duodenal biopsies were analyzed using culture and histopathology.

Main Results:

  • Mean H. pylori IgG and IgA antibody levels were significantly elevated.
  • H. pylori gastritis was confirmed histopathologically in 11 children.
  • Antral biopsy cultures were positive in 5 children, with one showing H. pylori-like organisms.

Conclusions:

  • Serological testing demonstrates high diagnostic accuracy for H. pylori infection in children.
  • Antibody detection is a valuable non-invasive method for diagnosing H. pylori in this population.
  • The findings support the use of serology as a primary diagnostic approach in Saudi Arabia.

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