Related Experiment Video
Updated: Aug 12, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
The major sigma factor (RpoD) from Helicobacter pylori and other gram-negative bacteria shows an enhanced rate of
J V Solnick1, L M Hansen, M Syvanen
1Department of Internal Medicine, School of Medicine, University of California, Davis 95616, USA. jvsolnick@ucdavis.edu
Abstract:
Sequence analysis of the Helicobacter pylori major sigma factor (RpoD) shows that it is highly divergent, which may be related to the marked diversity of the H. pylori chromosome. Furthermore, the rate of divergence of RpoD among other gram-negative bacteria is much greater than that among gram-positive bacteria. This suggests that RpoD from gram-negative bacteria is functionally less constrained than that from gram-positive bacteria.
More Related Videos
06:30Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography
Published on: June 4, 2019
08:24Using Mycobacterium smegmatis as a Bioindicator for Zinc-Limited Growth Conditions in Mycobacteria
Published on: September 20, 2024
Related Concept Videos
Replication in Prokaryotes
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Replication in Prokaryotes
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Stringent Response in E. coli
Other Stress Responses in Bacteria