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Related Experiment Videos

Postnatal development of low [Mg2+] oscillations in neocortex

A C Flint1, U S Maisch, A R Kriegstein

  • 1Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.

Journal of Neurophysiology
|October 27, 1997
PubMed
Summary

The development of intrinsically burst-firing (IB) neurons in rat somatosensory cortex influences the maturation of network rhythmic activity. This maturation is linked to changes in neuronal membrane properties and NMDA receptor function.

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Area of Science:

  • Neuroscience
  • Developmental Neuroscience
  • Cellular Electrophysiology

Background:

  • Neocortical rhythmic activity can be induced by unblocking N-methyl-D-aspartate (NMDA) receptors.
  • Intrinsically burst-firing (IB) neurons in cortical layer 5 are hypothesized to contribute to this rhythmic activity.

Purpose of the Study:

  • To investigate the developmental emergence of IB neurons in rat somatosensory cortex.
  • To examine the postnatal development of NMDA receptor-dependent rhythmic network activity.

Main Methods:

  • Whole-cell patch-clamp recordings from neonatal rat somatosensory cortex slices.
  • Field-potential recordings in low magnesium ([Mg2+]o) conditions.
  • Pharmacological manipulation using NE to inhibit IB cell burst-firing.

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Main Results:

  • IB neuron properties were absent before postnatal day 12 (P12) and increased progressively thereafter.
  • Low [Mg2+]o-induced rhythmic activity evolved from single action potentials at P7 to organized bursts (4-12 Hz) at P19.
  • Pharmacological inhibition of IB cells altered the pattern of network oscillations at P19.

Conclusions:

  • The maturation of layer 5 IB neurons is critical for the development of NMDA receptor-dependent rhythmic network activity in the neocortex.
  • Changes in intrinsic neuronal membrane properties play a key role in shaping network oscillations during postnatal development.