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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
Pertactin antigens extracted from Bordetella pertussis and Bordetella bronchiseptica differ in the isoelectric point
C Pagliaccia1, R Manetti, R Rappuoli
1IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina 1, 53100 Siena, Italy.
Abstract:
Pertactin, which is a membrane-associated antigen of Bordetella pertussis and which is present in many acellular vaccines against whooping cough, has been reported to be similar to the homologous protein in Bordetella bronchiseptica. By running parallel experiments using proteins derived from the two species, we show that the isoelectric point of pertactin from B. pertussis is lower than reported and clearly distinguishable from the homologous protein of B. bronchiseptica.

