Suppression of luteinizing hormone release by 5alpha-androstane-3alpha, 17 beta-diol and its 3 beta epimer in...

The effect of 5alpha-androstane-3alpha, 17 beta-diol, its 3 beta epimer and oestradiol benzoate on suppression of LH release after ovariectomy was studied in immature rats. At doses of 50 and 100 mug/100 g body weight/day the 3alpha compound suppressed LH release after ovariectomy to the same extent as 0-1 mug oestradiol benzoate/100 g/day. 3alpha-Androstanediol at a dose of 25 mug/100 g/day suppressed LH release only up to day 45 of life, while the same dose of the 3beta epimer had no effect on LH suppression. The effect of 3beta-androstanediol on inducing precocious vaginal opening was found to be mediated by the ovaries, since it was eliminated by ovariectomy. These results confirm our previous findings on the participation of androstanediol in the normal regulation of LH and in the mechanism of onset of puberty in the rat.