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CTL responses induced by a single immunization with peptide encapsulated in biodegradable microparticles
C D Partidos1, P Vohra, D Jones
1Department of Pathology and Infectious Diseases, Royal Veterinary College, London, UK. hpartido@rvc.ac.uk
Journal of Immunological Methods
|August 7, 1997
Summary
Poly (D,L-lactide co-glycolide) (PLG) microparticles effectively enhance cytotoxic T cell (CTL) responses to measles virus (MV) peptides. A single immunization with PLG-encapsulated peptides induced a robust CTL response in mice.
Area of Science:
- Immunology
- Vaccinology
- Materials Science
Background:
- Measles virus (MV) poses a significant global health challenge, necessitating effective vaccine strategies.
- Cytotoxic T cell (CTL) responses are crucial for viral clearance and long-term immunity.
- Poly (D,L-lactide co-glycolide) (PLG) is a biodegradable polymer widely used in drug delivery systems.
Purpose of the Study:
- To evaluate the immunogenicity of a synthetic measles virus (MV) cytotoxic T cell epitope (CTL) peptide encapsulated in poly (D,L-lactide co-glycolide) (PLG) microparticles.
- To compare the CTL response induced by PLG-encapsulated peptide with peptide administered in Freund's complete adjuvant.
- To investigate the potential of PLG microparticles as a delivery system for enhancing peptide-specific CTL responses.
Main Methods:
- Synthesis of a MV-specific CTL epitope peptide.
- Encapsulation of the peptide within 50:50 PLG microparticles.
- Intraperitoneal immunization of mice with varying doses of encapsulated peptide.
- Assessment of CTL responses through specific assays.
- Comparison with peptide administered in Freund's complete adjuvant and with a simple mixture formulation.
Main Results:
- A single intraperitoneal immunization with PLG-encapsulated MV peptide induced a strong CTL response in mice.
- The CTL response was superior to that achieved with peptide in Freund's complete adjuvant.
- A dose-dependent effect was observed, with 100 micrograms being more effective than lower doses.
- A vaccine formulation with empty PLG microparticles mixed with peptide elicited comparable CTL responses.
- A functional CTL response against MV-infected target cells was demonstrated.
Conclusions:
- PLG microparticles serve as an effective delivery system for enhancing peptide-specific CTL responses against measles virus.
- PLG microparticles possess inherent immunostimulatory properties that contribute to robust CTL priming.
- These findings support the potential of PLG microparticle-based vaccines for inducing strong and effective cell-mediated immunity against MV.