Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymorphism at codons 114, 116, 145, and 163 muddle the typing of HLA-B*1304

M Ellexson1, P Lai-Kwan, M Lau

  • 1Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.

Human Immunology
|June 1, 1997
PubMed
Summary

Genetic recombination in HLA-B*1304 causes misidentification as a B15 type by both serologic and molecular DNA typing methods. This study explains the molecular basis for this typing ambiguity in human leukocyte antigen (HLA) molecules.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The doctor in this new day.

Journal of the Medical Association of Georgia·2010
Same author

A phase I study of lenalidomide in combination with fludarabine and rituximab in previously untreated CLL/SLL.

Leukemia·2010
Same author

The novel protein BboRhop68 is expressed by intraerythrocytic stages of Babesia bovis.

Parasitology international·2010
Same author

A systematic review on animal models of maternal high fat feeding and offspring glycaemic control.

International journal of obesity (2005)·2010
Same author

Multisensory determinants of orientation perception: task-specific sex differences.

The European journal of neuroscience·2010
Same author

Changing concepts of homosexuality in psychoanalysis.

Psychiatry·2010

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Human Leukocyte Antigen (HLA) Typing

Background:

  • Human Leukocyte Antigen (HLA) class I typing is crucial for transplantation and disease association studies.
  • Genetic variations and recombination events can complicate accurate HLA typing.
  • HLA-B*1304 is known to present typing discrepancies between serologic and molecular methods.

Purpose of the Study:

  • To investigate the reasons behind the misclassification of HLA-B*1304 as a B15 type.
  • To analyze the molecular and serologic characteristics that lead to this typing ambiguity.
  • To understand the impact of genetic recombination on HLA typing accuracy.

Main Methods:

  • Comparative analysis of HLA-B13 and HLA-B15 families.
  • Examination of amino acid and nucleotide sequences of HLA-B*1304.

Related Experiment Videos

  • Analysis of serologic cross-reactivity patterns.
  • Evaluation of Polymerase Chain Reaction (PCR) amplification patterns in molecular typing.

    • Main Results:

    • HLA-B*1304 differs from other HLA-B13 molecules by only a few amino acids.
    • Genetic recombination at codons 145 and 163 causes B*1304 to show B15X21 serologic cross-reactivity.
    • B15-like sequences at residues 114, 116, and 145 result in a B15 PCR amplification pattern.
    • Both serologic and molecular DNA typing methods incorrectly identify B*1304 as a B15 type.

    Conclusions:

    • Genetic exchanges in key amino acid positions of the HLA class I heavy chain are responsible for the misclassification of HLA-B*1304.
    • The study highlights the challenges in HLA typing due to complex genetic variations.
    • Accurate HLA typing requires careful consideration of sequence-based analyses to resolve ambiguities.