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Related Experiment Videos

Polymorphonuclear leukocytes released from the bone marrow preferentially sequester in lung microvessels

S F van Eeden1, Y Kitagawa, M E Klut

  • 1University of British Columbia, Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, Canada.

Microcirculation (New York, N.Y. : 1994)
|November 5, 1997
PubMed
Summary

Endotoxemia prompts the bone marrow to release polymorphonuclear leukocytes (PMNs). These PMNs are less deformable and preferentially sequester in lung microvessels, impacting inflammatory responses.

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Area of Science:

  • Immunology
  • Hematology
  • Pulmonary Medicine

Background:

  • Polymorphonuclear leukocytes (PMNs) are critical in the systemic inflammatory response.
  • The release of PMNs from bone marrow is a hallmark of inflammation.
  • Understanding PMN sequestration in the lungs is vital for inflammatory disease research.

Purpose of the Study:

  • To quantify PMN release from bone marrow following endotoxin administration.
  • To determine the extent of PMN sequestration within the lung microvasculature.
  • To investigate the characteristics of endotoxin-induced PMN release and lung sequestration.

Main Methods:

  • Utilized 5'-bromo-2-deoxyuridine (BrdU) to label dividing PMNs in rabbit bone marrow.
  • Employed immunohistochemistry and morphometry to track BrdU-labeled PMNs in circulation and lung.

Related Experiment Videos

  • Measured circulating PMN counts, band cells, and arteriovenous differences across the lung.

    • Main Results:

    • Endotoxin induced a transient drop in circulating PMNs, followed by neutrophilia and increased band cells.
    • A significant increase in BrdU-labeled PMNs was observed in circulation and preferentially sequestered in the lung.
    • Endotoxin-treated rabbits showed higher percentages of BrdU-labeled PMNs in alveolocapillary walls and impaired deformability.

    Conclusions:

    • Endotoxemia stimulates the bone marrow to release both mature and immature PMNs.
    • These endotoxin-induced PMNs exhibit reduced deformability compared to normally released PMNs.
    • Preferential sequestration of these less deformable PMNs occurs within the lung microvasculature.