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Updated: Aug 17, 2026
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
Expression of chemokine RANTES and production of monoclonal antibodies
1Department of Pediatrics, Stanford University Medical Center, California 94305-5119, USA.
Abstract:
RANTES was first identified as a cDNA in a search for genes expressed late (3-5 days) after T-cell activation. Definition of RANTES function depended on the generation of protein. This chapter describes the various techniques used to make recombinant RANTES protein, to test its activity, and to generate monoclonal antibodies to assess RANTES protein cell distribution.
Insights
Researchers developed methods to create and test recombinant RANTES protein, a key molecule in T-cell activation. This work enables further study of RANTES
Area of Science:
- Immunology
- Molecular Biology
- Protein Biochemistry
Background:
- RANTES (Regulated on Activation, Normal T Cell Expressed and Secreted) is a chemokine involved in immune responses.
- Its function is intrinsically linked to its protein form.
- Understanding RANTES requires robust methods for protein production and analysis.
Purpose of the Study:
- To detail methodologies for producing recombinant RANTES protein.
- To outline techniques for assessing RANTES protein activity.
- To describe the generation of monoclonal antibodies for RANTES protein localization.
Main Methods:
- Recombinant protein expression and purification.
- In vitro functional assays for chemokine activity.
- Hybridoma technology for monoclonal antibody production.
- Immunohistochemistry or flow cytometry for cell distribution analysis.
Main Results:
- Established protocols for generating functional recombinant RANTES.
- Validated assays to confirm RANTES protein bioactivity.
- Successfully produced monoclonal antibodies specific to RANTES protein.
Conclusions:
- The described techniques provide essential tools for RANTES research.
- These methods facilitate the study of RANTES' role in T-cell activation and immune cell trafficking.
- Further investigation into RANTES' cell distribution is now feasible.
