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Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
Published on: December 12, 2014
Biological activity C-X-C and C-C chemokines on leukocyte subpopulations in human whole blood
1Department of Cancer, Immunology and Infectious Diseases, Pfizer, Inc., Groton, Connecticut 06340, USA.
Methods in Enzymology
|January 1, 1997
Abstract:
We have described an assay that monitors the activating effects of a variety of chemokines on leukocyte subsets in human whole blood. This procedure has the following advantages: (1) minimal manipulation of the cells, (2) maintenance of more physiological conditions, and (3) simultaneous monitoring of the responses of monocytes, neutrophils, and eosinophils.

