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Inhibition of virus-induced age-dependent poliomyelitis by interferon-gamma

W A Cafruny1, T R Haven, S R Lawson

  • 1Department of Microbiology, University of South Dakota School of Medicine, Vermillion, USA. bcafruny@sundance.usd.edu

Antiviral Research
|October 23, 1997
PubMed

Insights

Interferon-gamma (IFN-gamma) prevents age-dependent poliomyelitis (ADPM) in mice by inhibiting lactate dehydrogenase-elevating virus (LDV) replication in the spinal cord. This cytokine treatment offers protection without affecting anti-LDV immunity or viremia.

Area of Science:

  • Virology
  • Immunology
  • Neuroscience

Background:

  • Age-dependent poliomyelitis (ADPM) is a neuroparalytic condition caused by co-infection with lactate dehydrogenase-elevating virus (LDV) and murine leukemia virus (MuLV).
  • Understanding the mechanisms of ADPM pathogenesis and potential therapeutic interventions is crucial for managing neuroinflammatory diseases.

Purpose of the Study:

  • To investigate the therapeutic effects of interferon-gamma (IFN-gamma) on ADPM incidence.
  • To assess the impact of IFN-gamma on LDV and MuLV replication and anti-LDV immune responses.

Main Methods:

  • Treatment of ADPM-susceptible C58/M mice with IFN-gamma.
  • Monitoring of ADPM incidence, viral RNA expression in spinal cords, and IgG anti-LDV antibody levels.
  • Quantification of LDV and MuLV RNA in the central nervous system.

Main Results:

  • IFN-gamma treatment protected mice from paralytic ADPM.
  • Protection correlated with reduced LDV RNA expression in the spinal cord, but not MuLV RNA.
  • IFN-gamma did not affect LDV viremia or the IgG anti-LDV immune response.

Conclusions:

  • Spinal cord LDV replication is the critical determinant of ADPM.
  • Cytokine-mediated inhibition of LDV replication in the CNS can prevent neuroparalytic disease.
  • IFN-gamma represents a potential therapeutic strategy for ADPM by targeting viral replication in the CNS.

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