Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vertebrates have conserved capping protein alpha isoforms with specific expression patterns

M C Hart1, Y O Korshunova, J A Cooper

  • 1Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. mhart@cellbio.wustl.edu

Cell Motility and the Cytoskeleton
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The immunological synapse and the actin cytoskeleton: molecular hardware for T cell signaling.

Nature immunology·2001
Same author

TOR signaling regulates microtubule structure and function.

Current biology : CB·2000
Same author

Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae.

The Journal of cell biology·2000
Same author

Molecular switches in lipid rafts.

Nature·2000
Same author

Role of actin and Myo2p in polarized secretion and growth of Saccharomyces cerevisiae.

Molecular biology of the cell·2000
Same author

Pulmonary fibrosis: pathways are slowly coming into light.

American journal of respiratory cell and molecular biology·2000
Same journal

Mechanics and dynamics of the cytoskeleton. Proceedings of a FEBS workshop. June 22-26, 2008. Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Mechanics and dynamics of the cytoskeleton: a special issue stemming from the 2008 ECF Meeting/ FEBS Workshop (Mechanics and Dynamics of the Cytoskeleton) in Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Mechanics and dynamics of the cytoskeleton: a special issue stemming from the 2008 ECF Meeting/FEBS Workshop (Mechanics and Dynamics of the Cytoskeleton) in Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture.

Cell motility and the cytoskeleton·2009
Same journal

Centriole symmetry: a big tale from small organisms.

Cell motility and the cytoskeleton·2009
Same journal

Fluorescence-based quantitative scratch wound healing assay demonstrating the role of MAPKAPK-2/3 in fibroblast migration.

Cell motility and the cytoskeleton·2009
See all related articles

Vertebrates possess two conserved actin capping protein alpha isoforms (alpha1 and alpha2) with distinct roles. These isoforms show varied expression across tissues, suggesting specialized functions in cellular processes like motility.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Actin capping protein (CP) is vital for actin dynamics and cell motility.
  • Lower organisms have single actin capping protein alpha and beta subunits.
  • Chickens exhibit two similar alpha-subunit isoforms, hinting at vertebrate complexity.

Purpose of the Study:

  • To investigate the prevalence and conservation of multiple alpha-subunit isoforms in vertebrates.
  • To analyze the sequence conservation and phylogenetic relationships of actin capping protein alpha isoforms.
  • To explore the functional implications of distinct alpha-subunit isoform expression.

Main Methods:

  • Isolation and analysis of alpha-subunit complementary DNA (cDNA) from mice and humans.
  • Phylogenetic analysis of alpha-subunit isoform sequences.

Related Experiment Videos

  • Examination of alpha-subunit isoform expression in mouse cells and tissues.

    • Main Results:

    • Mice and humans possess two distinct alpha-subunit isoforms, designated alpha1 and alpha2.
    • Phylogenetic analysis confirms two highly conserved alpha-subunit subfamilies (alpha1 and alpha2) in vertebrates.
    • Differential expression observed: endothelial cells predominantly express alpha2, erythrocytes alpha1, and other tissues show variable ratios.

    Conclusions:

    • Vertebrates have evolved two conserved actin capping protein alpha isoforms with potentially unique functions.
    • Key amino acid differences distinguish the alpha1 and alpha2 subfamilies.
    • Tissue-specific expression patterns suggest specialized roles for each alpha-subunit isoform in vertebrate physiology.