Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation of osteosarcoma-associated amplified DNA sequences using representational difference analysis

A Simons1, I M Janssen, R F Suijkerbuijk

  • 1Department of Human Genetics, University Hospital, Nijmegen, The Netherlands. A.Simons@antrg.azn.nl

Genes, Chromosomes & Cancer
|October 23, 1997
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Multi-family therapy for adolescents with anorexia nervosa].

Tijdschrift voor psychiatrie·2023
Same author

Corrigendum to "Urinary incontinence and erectile dysfunction in patients with localized or locally advanced prostate cancer: A nationwide observational study".

Urologic oncology·2022
Same author

Initial management of blunt and penetrating neck trauma.

BJA education·2021
Same author

Specific proteome changes in platelets from individuals with GATA1-, GFI1B-, and RUNX1-linked bleeding disorders.

Blood·2021
Same author

Histopathological re-evaluations of biopsies in prostate cancer: a nationwide observational study.

Scandinavian journal of urology·2020
Same author

Urinary incontinence and erectile dysfunction in patients with localized or locally advanced prostate cancer: A nationwide observational study.

Urologic oncology·2020

Researchers identified amplified DNA regions in osteosarcoma tumors, specifically at chromosome 19. These findings aid in discovering genes crucial for osteosarcoma development.

Area of Science:

  • Genomics
  • Cancer Biology
  • Molecular Oncology

Background:

  • Osteosarcoma is a primary bone cancer with a propensity for metastasis.
  • Understanding genetic alterations in osteosarcoma is key to identifying therapeutic targets.

Observation:

  • Comparative genomic hybridization revealed DNA amplifications at 17p11.2-12 and 19q12-13 in osteosarcoma.
  • Representational difference analysis isolated tumor-amplified DNA fragments from chromosome 19.

Findings:

  • A YAC clone localized to 19q12-13.1 confirmed DNA amplification in osteosarcoma tumors.
  • Two distinct tumor-amplified DNA fragments were identified originating from chromosome 19.

Implications:

  • The isolated DNA sequences can facilitate the search for genes involved in osteosarcoma development.

Related Experiment Videos

  • This research provides valuable genetic markers for osteosarcoma research and potential therapeutic strategies.