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Cloning and analysis of cDNA encoding murine pinin
P Ouyang1, Y Y Zhen, S P Sugrue
1Department of Anatomy, Chang Gung College of Medicine and Technology, Tau-Yuan, Taiwan, R.O.C. ouyang@cguaplo.cgu.edu.tw
Gene
|October 23, 1997
Summary
Researchers identified the full-length mouse pinin gene, crucial for stabilizing cell junctions. This study details its genetic sequence and protein domains, offering insights into epithelial tissue structure and function.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Pinin is a protein associated with cell junctions.
- It plays a role in stabilizing the desmosome-intermediate filament complex.
- This complex is vital in various epithelial tissues.
Purpose of the Study:
- To isolate and characterize the full-length murine pinin gene.
- To analyze the genetic sequence and protein structure of mouse pinin.
- To compare mouse pinin with its canine and human counterparts.
Main Methods:
- Utilized a cDNA probe from canine pinin to isolate overlapping cDNA clones.
- Determined the nucleotide sequence of the murine full-length pinin cDNA.
- Predicted the amino acid sequence and identified protein domains.
Main Results:
- Isolated cDNA clones encoding full-length mouse pinin (725 amino acids).
- Mouse pinin shares high sequence identity with canine and human pinin, with a 38-amino acid deletion noted in mice.
- Identified distinct domains: three coiled-coil, glycine loops, glutamine-proline-rich, and polyserine domains.
Conclusions:
- The study provides the complete genetic and predicted protein sequence of mouse pinin.
- Mouse pinin exhibits conserved and divergent features compared to other species.
- Characterization of pinin's domains offers structural insights into its function in epithelial tissues.
