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[Receptor mediated gene transfer in squamous epithelial carcinoma of the head-neck area]
B Wollenberg1, W Schmidt, B Schmitt
1Klinik und Poliklinik für Hals-Nasen-Ohren-Heilkunde und Kopf-Hals-Chirurgie, Ludwig-Maximilians-Universität München.
Background:
The "Adeno-Virus-Enhanced-Receptor-Mediated" (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced.
Methods:
Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reportergene for luciferase with either AVET or different liposoms in vitro.
Results:
In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied.
Conclusions:
AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.