Related Experiment Videos
[Effect of promethazine hydrochloride (pipolphen) on the stability of R plasmid resistance in Escherichia coli]
Abstract:
The influence of promethazin hydrochloride (pipolphen) on stability of R plasmid inheritance in Escherichia coli strains of various serogroups was studied. The strains were isolated from patients with acute intestinal infection and from healthy persons. It was shown that in subbacteriostatic concentrations (100 to 450 micrograms/ml) pipolphen promoted elimination of the R plasmids. Decreased stability of the R plasmid inheritance was not associated with the pipolphen concentration. No influence of the drug on the biochemical characteristics, antigenic properties and nutritional requirements of the plasmid-free derivatives was detected. The eliminating action of pipolphen and ethidium bromide in some strans of Escherichia coli was shown to be different.
Insights
Promethazine hydrochloride (pipolphen) at low concentrations can eliminate R plasmids from Escherichia coli strains. This drug
Area of Science:
- Microbiology
- Molecular Biology
- Pharmacology
Background:
- R plasmids confer antibiotic resistance in bacteria.
- Escherichia coli is a common cause of intestinal infections.
- Understanding plasmid stability is crucial for combating antibiotic resistance.
Purpose of the Study:
- To investigate the effect of promethazine hydrochloride (pipolphen) on R plasmid inheritance stability in Escherichia coli.
- To determine if pipolphen can eliminate R plasmids from bacterial strains.
Main Methods:
- Studied Escherichia coli strains from infected patients and healthy individuals.
- Assessed the impact of sub-bacteriostatic concentrations of pipolphen (100-450 µg/ml) on R plasmid stability.
- Compared the eliminating action of pipolphen with ethidium bromide.
Main Results:
- Pipolphen promoted the elimination of R plasmids in Escherichia coli at sub-bacteriostatic concentrations.
- The degree of R plasmid inheritance instability was not directly correlated with pipolphen concentration.
- Plasmid-free derivatives showed no changes in biochemical characteristics, antigenic properties, or nutritional requirements.
- Pipolphen exhibited a different eliminating action compared to ethidium bromide in some strains.
Conclusions:
- Promethazine hydrochloride (pipolphen) can act as an R plasmid-eliminating agent in Escherichia coli.
- Pipolphen's effect on plasmid stability is concentration-independent within the tested range.
- The mechanism of pipolphen-induced plasmid elimination differs from that of ethidium bromide.