Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Activity-dependent regulation of NMDAR1 immunoreactivity in the developing visual cortex

S M Catalano1, C K Chang, C J Shatz

  • 1Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|October 23, 1997
PubMed
Summary

High levels of NMDAR1, a key component of NMDA receptors, in the developing visual cortex are linked to synaptic plasticity. Retinal activity is crucial for maintaining these levels during critical developmental periods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ASO Visual Abstract: Impact of Mainstream Germline Genetic Testing with Expanded Eligibility for Early Stage Breast Cancer Patients in a Large Integrated Health System.

Annals of surgical oncology·2024
Same author

Mainstream Germline Genetic Testing with Expanded Eligibility for Early Breast Cancer Patients in a Large Integrated Health System.

Annals of surgical oncology·2024
Same author

[Mechanisms by which the bone marrow microenvironment of myelodysplastic neoplasms contributes to disease progression].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2023
Same author

[Potential application of improved hard tissue section technique in the clinical pathological diagnosis of bone and bone marrow].

Zhonghua yi xue za zhi·2022
Same author

A Multicenter Virtual Multidisciplinary Sarcoma Case Conference Improves Outcome for a Rare Soft Tissue Sarcoma (Dermatofibrosarcoma Protuberans).

The American surgeon·2022
Same author

[Influencing factors of iron metabolism assessment in patients with myelodysplastic syndrome: A retrospective study].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2022

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Visual System Research

Background:

  • NMDA receptors (NMDAR1) are crucial for activity-dependent synaptic plasticity in the developing visual cortex.
  • Understanding the developmental regulation of NMDAR1 is key to comprehending critical period plasticity.

Purpose of the Study:

  • To investigate the developmental distribution of NMDAR1 in the visual cortex of cats and ferrets.
  • To determine the role of retinal activity in regulating NMDAR1 levels during visual cortex development.

Main Methods:

  • Immunocytochemical detection of NMDAR1 in visual cortex across developmental stages (embryonic to adult).
  • Experimental manipulation including dark-rearing and monocular blockade of retinal activity using tetrodotoxin (TTX).

Related Experiment Videos

Main Results:

  • NMDAR1 immunostaining is initially high in cortical neurons, declining with development, except in layers 2/3 where it remains high.
  • Declining NMDAR1 in layer 4 correlates with ocular dominance column formation and the end of the critical period.
  • Monocular TTX treatment significantly decreases NMDAR1 immunoreactivity in layer 4 of the affected eye's columns.
  • Dark-rearing did not alter NMDAR1 immunoreactivity levels.

Conclusions:

  • High NMDAR1 levels in the visual cortex correlate with ocular dominance column formation and developmental plasticity.
  • Visual experience is not essential for developmental changes in NMDAR1 laminar patterns.
  • Retinal activity is required for maintaining high NMDAR1 levels in layer 4 during the critical period.
  • NMDA receptors play a central role in both promoting and limiting synaptic rearrangements in the developing neocortex.