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Stoichiometry of the interaction of prostaglandin H synthase with substrates
1Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, Texas 77030, USA.
Biochemistry
|October 23, 1997
Abstract:
Prostaglandin H synthase (PGHS) catalyzes both peroxidase and cyclooxygenase reactions. Resolution of several current issues regarding the PGHS catalytic mechanism hinges on the stoichiometry of the reaction of PGHS with hydroperoxide, fatty acid, and oxygen. The dependence of wide-doublet tyrosyl radical accumulation in PGHS isoform 1 on hydroperoxide stoichiometry, has been determined; this catalytically active radical is formed efficiently at stoichiometries