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pH-dependent specific binding and combing of DNA

J F Allemand¹, D Bensimon, L Jullien

¹LPS, ENS, URA D 1306 CNRS, associé aux universitié Paris VI, France.

Biophysical Journal

|October 23, 1997

Summary

Specific DNA binding to surfaces is achieved by controlling pH. This pH-dependent method enhances DNA end-binding, enabling precise molecular combing for DNA analysis.

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Area of Science:

Biophysics
Molecular Biology
Surface Chemistry

Background:

Rapid DNA sequencing and mapping require specific DNA-surface interactions.
Current methods often depend on specially treated surfaces for DNA binding.

Purpose of the Study:

To demonstrate specific DNA binding to diverse surfaces using pH control.
To investigate the pH-dependent binding mechanism and its application in DNA manipulation.

Main Methods:

Utilizing a pH-controlled approach for DNA adsorption onto various surfaces.
Employing a model to explain differential binding of DNA extremities versus midsegments.
Applying molecular combing technique using a receding meniscus.

Main Results:

Specific DNA binding via unmodified ends achieved on multiple surfaces by adjusting pH.
Optimal binding efficiency on hydrophobic surfaces observed at pH ~5.5.
At pH 5.5, DNA molecule ends are significantly more likely to bind than internal segments.

Conclusions:

pH-dependent DNA binding offers a versatile method for achieving specific end-attachment.
This technique facilitates the alignment of DNA molecules for advanced analysis, such as molecular combing.
The proposed model accurately describes the pH-driven differential adsorption of DNA.