Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Driving proteins off DNA using applied tension

J F Marko1, E D Siggia

  • 1Department of Physics, The University of Illinois at Chicago, 60607-7059, USA. marko@isolda.phy.uic.edu

Biophysical Journal
|October 23, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanical unfolding of spectrin reveals a super-exponential dependence of unfolding rate on force.

Scientific reports·2019
Same author

Author Correction: Self-organization of a human organizer by combined Wnt and Nodal signalling.

Nature·2018
Same author

Self-organization of a human organizer by combined Wnt and Nodal signalling.

Nature·2018
Same author

Stochastic ratchet mechanisms for replacement of proteins bound to DNA.

Physical review letters·2014
Same author

Forced periodic expression of G1 cyclins phase-locks the budding yeast cell cycle.

Proceedings of the National Academy of Sciences of the United States of America·2009
Same author

Cross-species comparison of Drosophila male accessory gland protein genes.

Genetics·2005
Same journal

Heterogeneous binding of SARS-CoV2 fusion peptide on complex cellular membranes enhances its fusogenicity.

Biophysical journal·2026
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
See all related articles

Applying force to DNA-binding proteins like histones can cause dissociation. Tensions greater than 2 piconewtons are predicted to detach histone octamers from chromatin fiber, impacting DNA structure.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Genetics

Background:

  • Proteins binding to DNA can alter its physical properties, including its end-to-end length.
  • Chromatin fiber, composed of DNA and histone proteins, forms the basic structural unit of eukaryotic chromosomes.

Purpose of the Study:

  • To investigate the thermodynamic principles governing the dissociation of DNA-binding proteins under applied force.
  • To specifically analyze the force-induced dissociation of histone octamers from chromatin fiber.

Main Methods:

  • Thermodynamic analysis of protein-DNA interactions.
  • Modeling of force-induced dissociation of histone octamers from DNA.

Main Results:

  • Proteins that compact DNA can be removed by applying external force.

Related Experiment Videos

  • The histone octamer is predicted to dissociate from the chromatin fiber at tensions exceeding 2 piconewtons.

    • Conclusions:

    • Mechanical force is a viable mechanism for disrupting histone-DNA interactions.
    • Understanding these force-dependent dynamics is crucial for comprehending chromatin organization and DNA accessibility.