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The GT-rich sequence in the U5 region of Rous sarcoma virus long terminal repeat is required for transcription
P J Cleavinger1, J C Kandala, R V Guntaka
1Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri-Columbia 65212, USA.
Abstract:
The sequences in the LTR of Rous sarcoma virus that are required for transcription termination and polyadenylation have been determined. A vector containing LTR-neo-LTR has been constructed and deletions in the U5 region of the downstream LTR have been made. The DNAs from wild-type and deletion mutant recombinant plasmids were introduced into QT6 cells and G418-resistant transformants were selected. Those transformants with neo sequences in the arrangement, LTR-neo-LTR, were analyzed for transcription termination and polyadenylation by Northern blot analysis and by S1 protection experiments. The results indicate that the polyadenylation signal, AATAAA, located in the U3 region alone, is not sufficient for 3' end processing and that the sequence between +20 and +44 in the U5 region is absolutely required for transcription termination or endonucleolytic cleavage and polyadenylation.