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Effect of human hydrosalpinx fluid on murine embryo development and implantation
1Greater Baltimore Fertility Center, Department of Gynecology, Greater Baltimore Medical Center, Maryland, USA.
Objective:
To determine the effect of hydrosalpinx fluid-containing medium on murine embryo development and implantation.
Design:
The development of one-, two-, and four-cell mouse embryos in medium containing 5%, 10%, and 20% of human hydrosalpinx fluid was observed. Implantation rates of mouse embryos transferred into the uterine horn with hydrosalpinx fluid-containing media were determined.
Setting:
Private hospital-based fertility center and IVF program.
Main Outcome Measure(S):
Percentage of embryos continuing cell division and implantation rates after ET.
Result(S):
Hydrosalpinx fluid in culture medium affected embryo development in a dose-dependent fashion. The injection of hydrosalpinx fluid-containing medium into the uterine horn did not affect embryo implantation.
Conclusion(S):
Hydrosalpinx fluid negatively affects murine embryo development, but its presence in the uterine horn at ET did not affect implantation.