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Use of a newly developed rapid microbial ATP bioluminescence assay to detect microbial contamination on poultry
G R Siragusa1, W J Dorsa, C N Cutter
1United States Department of Agriculture, Agricultural Research Service (USDA-ARS), Roman L. Hruska US Meat Animal Research Center, Clay Center, NE 68933, USA.
Abstract:
A newly developed rapid microbial ATP bioluminescence test (R-mATP) was shown to be an adequate means to assay the microbial load of poultry carcasses. This assay utilizes differential extraction and filtration to separate somatic from microbial ATP in a very rapid timeframe. The assay requires approximately 5 min to complete; approximately 3.5 min to sample and 90 s analytical time. Correlation coefficient (r) between aerobic colony counts and R-mATP test results (n=329) was 0.82. Post-test probabilities to correctly classify carcasses with different levels of microbial contamination were as high as 98% for samples of > or = 3.5 log aerobic CFU per ml. Given the rapidity of this assay, the R-mATP holds potential for monitoring the microbial load of carcasses at poultry-processing critical control points. Other potential applications of this new version of the microbial ATP bioluminescence test are discussed.
Insights
A new rapid microbial ATP bioluminescence test (R-mATP) accurately assesses poultry carcass microbial load in under 5 minutes. This fast, reliable method can monitor contamination at critical processing points.
Area of Science:
- Food Science
- Microbiology
- Analytical Chemistry
Background:
- Accurate microbial load assessment is crucial for poultry safety.
- Traditional methods are time-consuming, limiting real-time monitoring.
- Rapid testing is needed for critical control points in poultry processing.
Purpose of the Study:
- To evaluate a novel rapid microbial ATP bioluminescence test (R-mATP) for quantifying microbial contamination on poultry carcasses.
- To determine the correlation between R-mATP results and traditional aerobic colony counts.
- To assess the potential of R-mATP for real-time monitoring in poultry processing.
Main Methods:
- Development of a rapid microbial ATP bioluminescence test (R-mATP) using differential extraction and filtration.
- Analysis of 329 poultry carcass samples.
- Comparison of R-mATP results with aerobic colony counts (CFU/ml).
Main Results:
- The R-mATP test demonstrated high accuracy in assaying microbial load.
- A strong correlation (r=0.82) was observed between R-mATP results and aerobic colony counts.
- High post-test probabilities (up to 98%) for correct classification of contamination levels were achieved.
Conclusions:
- The R-mATP test is an adequate and rapid method for microbial load assessment of poultry carcasses.
- Its speed and accuracy make it suitable for monitoring critical control points in poultry processing.
- The R-mATP test shows promise for improving food safety monitoring in the poultry industry.