Optical resolution of DL-alanine by using immobilized Aspergillus oryzae cells

Mycelium pellets with diameters of 1-2 mm and containing abundant aminoacylase were obtained by the liquid fermentation of Aspergillus oryzae 3042. By the cross-linking method with reagents of gelatin and formaldehyde, the immobilized A. oryzae cells (IAC) were prepared with much higher activity and reactive properties. The effects of factors on enzymatic activity of IAC were investigated. When substrate concentration was less than 0.15 mol/L, the Michaelis-Menten mechanism was suitable for this reaction.