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High pressure modulation of Escherichia coli DNA gyrase activity
L N Chilukuri1, P A Fortes, D H Bartlett
1Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, La Jolla, California 92093, USA.
Biochemical and Biophysical Research Communications
|November 5, 1997
Abstract:
Elevated pressures greater than 551 bar were found to inhibit the DNA supercoiling activity of Escherichia coli DNA gyrase. A large fraction of the inhibitory effect could be reproduced by preincubation of the enzyme at high pressure prior to enzymatic assay at 1 bar. It is proposed that elevated pressure influences gyrase structure, most likely by promoting the dissociation of its subunits, however, it is also possible that effects on enzyme activity exist.