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Matrix glycoprotein SC1/ECM2 augments B lymphopoiesis
K Oritani1, Y Kanakura, K Aoyama
1Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA.
Blood
|November 14, 1997
Summary
Matrix glycoprotein SC1/ECM2 supports B-lymphocyte development. This study details its gene sequence and demonstrates SC1/ECM2’s role in augmenting B-cell precursor cloning and mature B-cell proliferation.
Area of Science:
- Immunology
- Developmental Biology
- Molecular Biology
Background:
- Stromal cell-derived extracellular matrix is crucial for lympho-hematopoiesis.
- Matrix glycoprotein SC1/ECM2 is a component of this matrix, potentially supporting B-lymphocyte precursors.
Purpose of the Study:
- To characterize the murine SC1/ECM2 gene and protein.
- To investigate the role of SC1/ECM2 in B-lymphopoiesis.
Main Methods:
- cDNA sequencing and chromosomal localization of murine SC1/ECM2.
- Preparation and testing of SC1/ECM2-Ig fusion proteins.
- Assays for B-cell precursor cloning and mature B-cell proliferation.
- Expression of SC1/ECM2 as a transmembrane protein.
Main Results:
- Complete cDNA sequence and chromosome 5 localization of murine SC1/ECM2.
- SC1/ECM2 fusion protein recognizes pre-B cells and enhances B-cell precursor cloning and mature B-cell proliferation in a divalent cation-dependent manner.
- SC1/ECM2 augments lymphopoiesis when expressed as a transmembrane protein.
- The N-terminal portion of SC1/ECM2 is sufficient for augmenting lymphocyte growth.
Conclusions:
- SC1/ECM2 is a key regulator of B-lymphopoiesis.
- SC1/ECM2 influences both B-cell precursor development and mature B-cell function.
- The extracellular matrix glycoprotein SC1/ECM2 has significant implications for understanding immune cell development.